cd133 antibody  (Cell Signaling Technology Inc)

Journal: Scientific Reports

Article Title: A CXCR4 targeting peptide delivered by silica nanoparticles eliminates migrating cancer stem cells in pancreatic ductal adenocarcinoma

doi: 10.1038/s41598-026-48584-2

Figure Lengend Snippet: CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells and CD133 + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.

Article Snippet: Mouse CD133/1 IgG1κ (AC133) , PE , Miltenyi Biotec , 130-113-108.

Techniques: Expressing, Protein-Protein interactions, Cell Culture, Derivative Assay, Cytometry, Standard Deviation

Journal: Scientific Reports

Article Title: A CXCR4 targeting peptide delivered by silica nanoparticles eliminates migrating cancer stem cells in pancreatic ductal adenocarcinoma

doi: 10.1038/s41598-026-48584-2

Figure Lengend Snippet: BMI1 downstream CXCL12–CXCR4 regulates EMT and stemness. ( A ) Protein–protein interactions of CXCL12 and CXCR4 with relevant factors involved in metastasis (red), stemness (purple), sonic hedgehog signaling (blue), AKT signaling (grey) and NFκB pathway (yellow) (STRING). ( B ) BMI1 gene expression analysis and western blot analysis. GAPDH was used as a loading control. Intensity ratios (IR) calculated against control lane using ImageJ. Cropped blot for clarity. ( C ) Gene expression analysis with genes involved in EMT using qRT-PCR. ( D ) Immunofluorescence quantifications and representative micrographs for indicated cell lines with white arrowheads marking mesenchymal structures of actin filaments stained with Phalloidin (pink) and nucleus stained with DAPI (blue). ( E ) Migration assays towards serum containing media. ( F ) Experimental scheme to evaluate CD133 and CXCR4 surface expression using flow cytometry for Panc354 and MetPO1 (sh_ SCR , sh1_ BMI1 and sh2_ BMI1 ) with (or without) CXCL12. ( G ) Flow cytometry analysis of CD133 + cells and CD133 + CXCR4 + cells in MetPO1 (sh_ SCR , sh1_ BMI1 and sh2_ BMI1 ) treated with (or without) CXCL12. ( H ) Representative cytometry plots for MetPO1 cell line. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.

Article Snippet: Mouse CD133/1 IgG1κ (AC133) , PE , Miltenyi Biotec , 130-113-108.

Techniques: Protein-Protein interactions, Gene Expression, Western Blot, Control, Quantitative RT-PCR, Immunofluorescence, Staining, Migration, Expressing, Flow Cytometry, Cytometry, Standard Deviation